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Primers used in this study for 16S rDNA and ITS2 amplicon <t> sequencing. </t>
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Primers used in this study for 16S rDNA and ITS2 amplicon sequencing.

Journal: Frontiers in Plant Science

Article Title: The mycoparasite Pythium oligandrum induces legume pathogen resistance and shapes rhizosphere microbiota without impacting mutualistic interactions

doi: 10.3389/fpls.2023.1156733

Figure Lengend Snippet: Primers used in this study for 16S rDNA and ITS2 amplicon sequencing.

Article Snippet: The sequencing data were processed under R v3.4 ( www.r-project.org ) using the R-Studio ( http://www.rstudio.com/ ) Phyloseq package ( ).

Techniques: Amplification, Sequencing

Transcriptomic analysis of Pythium oligandrum -inoculated roots. RNAseq experiments were performed on RNA extracted from in vitro grown plants inoculated with P. oligandrum oospores at different time points. (A) Principal component analysis of data obtained from the RNA sequencing of P. oligandrum and A17 M. truncatula at 3, 5, 7, and 14 days post-inoculation. Orange dots show data belonging to M. truncatula plants inoculated with P. oligandrum , and green dots show data belonging to mock plants. (B) Number of differentially expressed genes (DEGs) at each time point. (C) Venn diagrams representing the DEGs for each condition. (D) Hierarchical clustering on DEGs; clustering was obtained using Hierachical Clustering Explorer version 3.5 with default parameters.

Journal: Frontiers in Plant Science

Article Title: The mycoparasite Pythium oligandrum induces legume pathogen resistance and shapes rhizosphere microbiota without impacting mutualistic interactions

doi: 10.3389/fpls.2023.1156733

Figure Lengend Snippet: Transcriptomic analysis of Pythium oligandrum -inoculated roots. RNAseq experiments were performed on RNA extracted from in vitro grown plants inoculated with P. oligandrum oospores at different time points. (A) Principal component analysis of data obtained from the RNA sequencing of P. oligandrum and A17 M. truncatula at 3, 5, 7, and 14 days post-inoculation. Orange dots show data belonging to M. truncatula plants inoculated with P. oligandrum , and green dots show data belonging to mock plants. (B) Number of differentially expressed genes (DEGs) at each time point. (C) Venn diagrams representing the DEGs for each condition. (D) Hierarchical clustering on DEGs; clustering was obtained using Hierachical Clustering Explorer version 3.5 with default parameters.

Article Snippet: The sequencing data were processed under R v3.4 ( www.r-project.org ) using the R-Studio ( http://www.rstudio.com/ ) Phyloseq package ( ).

Techniques: In Vitro, RNA Sequencing